Top seed Angelique Kerber will lose her world number one ranking after being beaten by Spain's Garbine Muguruza in the fourth round at Wimbledon.
Muguruza, the 14th seed and a runner-up at SW19 in 2015, won an enthralling match 4-6 6-4 6-4 in two hours and 20 minutes on court two.
The German, a finalist in 2016, had needed to reach Saturday's final to have a chance of retaining her ranking.
Muguruza, 23, will play Svetlana Kuznetsova of Russia in the last eight.
Big-hitting Muguruza was encouraged to go on the attack by her coach Conchita Martinez, the 1994 champion who is still the only Spanish woman to win Wimbledon.
The 14th seed's ferocious backhand began to misfire at a crucial time at the end of the first set but she continued her assault and was soon level.
Kerber's counter-punching style meant she was far steadier throughout but she eventually cracked at the end of a topsy-turvy deciding set.
The 29-year-old lost in the final here last year, but has had some miserable results in 2017 and will be replaced as number one by Romania's Simona Halep, 25, who plays Victoria Azarenka in the last 16, if she reaches the semi-finals.
If Halep does not reach the last four, 25-year-old Karolina Pliskova of the Czech Republic will move to top spot despite her second round exit at Wimbledon.