A judge in Pennsylvania has dismissed a lawsuit from the Trump campaign that sought to invalidate millions of mail-in votes in the battleground state.
Judge Matthew Brann said the suit, which rested on allegations of irregularities, was "without merit".
The move paves the way for Pennsylvania to next week certify Joe Biden's win - he leads by more than 80,000 votes.
It is the latest blow to Donald Trump, who is trying to overturn his loss in the 3 November presidential election.
He has refused to concede and made allegations of widespread electoral fraud, without providing any evidence.
The lack of a concession has upended the process that normally follows a US election.
Mr Biden is projected to defeat President Trump 306 to 232 in the US electoral system, which determines who becomes president - far above the 270 he needs to win.
The Trump campaign has lost a slew of lawsuits contesting results from the election, and their latest efforts focus on stopping the swing states that handed Mr Biden his win certifying the results - an essential step for the Democrat to be formally declared victor.
Pennsylvania judge gives scathing verdict
In the ruling Judge Brann wrote that the Trump campaign had tried to "disenfranchise almost seven million voters".
He said his "court has been presented with strained legal arguments without merit and speculative accusations".
"In the United States of America, this cannot justify the disenfranchisement of a single voter, let alone all the voters of its sixth most populated state," the judge wrote.
The Trump campaign argued that the state had violated the US Constitution's guarantee of equal protection under the law as some, Democratic-run counties allowed voters to fix errors on their ballots while Republican-run counties did not.
But in his ruling Judge Brann dismissed the claim, saying "like Frankenstein's Monster" it had been "haphazardly stitched together". He said even if it was the basis for a case then the Trump campaign's solution went too far.
Mr Trump's legal team announced it would urgently seek to appeal against the decision.
Few Republicans have called on the president to concede, but following the judge's ruling Republican Pennsylvania Senator Pat Toomey said Mr Trump had exhausted all legal options in the state and urged him to accept the result.
What's the situation in other battleground states?
Also on Saturday the Trump campaign called for another recount in Georgia, a day after a by-hand recount confirmed Mr Biden's win in the state.
The campaign said it "has to include signature matching and other vital safeguards".
In another swing state won by Mr Biden, Michigan, Republican officials wrote to the state's electoral board to request a two-week delay in certifying the results.
They called for an audit of the presidential vote in the largest county, home to Detroit, after it was contested by the Trump camp.
But the Michigan Department of State said delays and audits were not permitted by law.
In Wisconsin, election officials accused Trump supporters of obstructing the state's recount of votes. They said observers for Mr Trump were in some cases challenging every single ballot to deliberately slow down proceedings.
If the recount is not concluded by 1 December - the deadline for Wisconsin to certify its votes - the way is open for the Trump camp to pursue a lawsuit.
Mr Biden has a lead of more than 20,000 in the state.
Why is election certification important?
When Americans vote in a presidential election, they are actually voting in a state contest, not a national one.
They are voting for state electors who will then cast one vote each for president. These electors usually follow the will of the electorate - in Michigan, for instance, they should all vote for Joe Biden because he won the state.
The states get a varying number of votes, equivalent to the number of their representatives in the US Congress - the House and the Senate.
Formality or undue significance?
Analysis box by Anthony Zurcher, North America reporter
What is typically a mere formality during normal elections - the bipartisan certification of state vote totals - has become the latest battleground in the president's attempts to maintain power for the next four years.
Could Trump actually be successful? It's not impossible, but the chances are very, very slim. First of all, the president would have to overturn the results in multiple states, where Biden's leads range from tens of thousands of votes to more than a hundred thousand. This isn't 2000, when everything came down just to Florida.
What's more, many of the states Trump's legal team is targeting - Michigan, Wisconsin and Pennsylvania - have Democratic governors who are not going to sit idly by while all of this takes place.
That doesn't mean Biden's supporters aren't worried, however. While the odds of this happening are along the lines of the earth being hit by a giant meteor or someone getting struck by lightning while winning the lottery, having victory snatched away at this point would be such a cataclysmic political event that the remote prospect of such a possibility is enough to give Democrats cold sweats.